Biotechnology Principles And Processes - Botany - Biology Previous Year Questions

A 1000 base-pair DNA fragment was cloned between Hind III and EcoRI sites of the plasmid vector (plAT) of size 3500 base-pair. The cloned fragment had a Not I site as shown in the figure. In order to confirm the presence of the insert, the recombinant plasmid was digested completely with (a) Not I and EcoR I, and (b) Not I and Hind III.

In lane 1 the products of the digestion by Not I and EcoRI was loaded. In lane 2 the products of the digestion by Not I and Hind III was loaded. Which one of the following correctly represents the agarose gel electrophoresis profile of the digested recombinant plasmid for (a) and (b), respectively?

Which of the following plasmid vectors can be used for cloning of a gene, with restriction enzymes BamHI and EcoRI, and ampicillin-containing nutrient agar for selection? [Ori - origin of replication; $\mathrm{Amp}^R$ - gene for ampicillin resistance]

Polymerase chain reaction (PCR) is used to amplify a gene of interest (GOI). If, after 30 cycles of PCR, 1 billion copies of GOI are produced, approximately how many copies of GOI were present at the end of the $20^{\text {th }}$ cycle?

The given picture was obtained from an agarose gel electrophoresis of a plasmid after digestion with restriction enzymes either $\mathrm{X}, \mathrm{Y}$ or both X and Y.

Which one of the following diagrams correctly represents the position of the restriction enzyme sites $(\mathrm{X}, \mathrm{Y})$ on the 10,000 bp plasmid?