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NCERT Exemplar · Class 12 · Biology

Chapter 11: Biotechnology: Principles and Processes

Practice each question, then open the answer or worked solution.

Short Answer Questions

12 questions
Q11Written answer

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


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Ans: 

A: Denaturation- Strands of DNA are separated into single strands by breaking hydrogen bonds between base pairs.

B: Annealing- It is a heat treatment process of DNA in which a cycle of reaction of heating and cooling is done.

C: Extension- By using DNA polymerase copy of the DNA strand is made.


Q12Written answer

Name the regions marked A, B and C.


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Ans:


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A- shows tetracycline resistance site

B- shows restriction site Pst I

C- shows ampicillin resistance site