Biotechnology Principles And Processes - Botany - Biology Previous Year Questions

During PCR cycle, the temperature range in the step of annealing is________

The manipulation of genetic material towards a desired end using "in vitro" process is called _________.

Which one of the following is considered as molecular scissors in modern biotechnology?

Match the Column I containing restriction enzymes with Column II containing recognition sequences.

When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of _________ enzyme.

Agarose gel electrophoresis is used to separate DNA fragments. The DNA fragments separate due to __________ .

The restriction enzymes used as molecular scissors are type of ____________

Which of the following technique is used for invitro amplification of gene?

In tissue culture technique, to maintain aseptic conditions the nutrient medium is autoclaved at constant pressure of _________ $\mathrm{lb} / \mathrm{sq}$ inch for continuous __________ minutes.

The transfer of rDNA into a bacterial cell for the purpose of its cloning, is assisted by ________ ions.

Which one of the following is NOT a technique used for direct transfer of foreign DNA into the protoplast of host cell without using vector?

$$\text { Match the restriction enzyme in Column I with their recognition sequences in Column II. }$$

Given below are two statements.

Statement I - The recognition sites of RENs are 4-8 nucleotides bps long and are characterized by a particular type of internal symmetry.

Statement II - Different restriction enzymes found in different organisms recognise different nucleotide sequences and cut the DNA at different sites.

In the light of above statements, select the correct option given below:

What is the time duration required for completion of one PCR Cycle?

In genetic engineering, the foreign DNA is also called ___________ .