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Previous year question hub

Recombinant DNA technology - Recombinant DNA technology and Other Tools in Biotechnology - Biotechnology Previous Year Questions

Practice Recombinant DNA technology - Recombinant DNA technology and Other Tools in Biotechnology - Biotechnology previous year questions organised from real papers, with year-wise coverage and clear topic navigation.

9Papers
9Years
16Questions
1Topics

Recombinant DNA technology question pattern

Every graph below is calculated only from this selection.

Questions by year

Compare question counts across years.

Difficulty distribution

How the classified questions are distributed by difficulty.

Easy 8 50%
Medium 8 50%

Question type distribution

MCQ, numerical, multiple-select and other formats found in these papers.

MCQ 13 81.3%
MSQ 3 18.8%

Subject weightage

Top subjects by unique question coverage.

Biotechnology
16 Qs

Most asked topics

Top topics across the included previous year papers.

Recombinant DNA technology and Other Tools in Biotechnology
16 Qs

Subtopic coverage

Top subtopics inside this exact selection.

Recombinant DNA technology
16 Qs

Paper coverage

Question coverage for the most populated papers. Every active PYP paper remains listed below.

Biotechnology (BT) 2023
1 Qs
Biotechnology (BT) 2022
1 Qs
Biotechnology (BT) 2021
1 Qs
Biotechnology (BT) 2020
1 Qs
Biotechnology (BT) 2019
1 Qs
Biotechnology (BT) 2017
2 Qs
Biotechnology (BT) 2016
2 Qs
Biotechnology (BT) 2013
3 Qs
Biotechnology (BT) 2010
4 Qs

Included previous year papers

Newest papers appear first. Search these papers or sort by year and name.

Paper nameYearPDFAttempt
Biotechnology (BT) 20232023
1 questions in this view
2023
Biotechnology (BT) 20222022
1 questions in this view
2022
Biotechnology (BT) 20212021
1 questions in this view
2021
Biotechnology (BT) 20202020
1 questions in this view
2020
Biotechnology (BT) 20192019
1 questions in this view
2019
Biotechnology (BT) 20172017
2 questions in this view
2017
Biotechnology (BT) 20162016
2 questions in this view
2016
Biotechnology (BT) 20132013
3 questions in this view
2013
Biotechnology (BT) 20102010
4 questions in this view
2010

All Recombinant DNA technology previous year questions

Practice every matching question in batches of 20, with every available option.

1
2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2013
In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to
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2
2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2013
The minimum number of independent recombinant clones required to represent this fragment in genomic library are
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3
2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2013

The number of clones to represent this fragment in genomic library with a probability of 95% are

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4
2016 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2016

Select the CORRECT combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.

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5
2016 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2016

A 1.2 kb DNA fragment was cloned into BamHI and EcoRI sites located on a 2.8 kb cloning vector. The BamHI and EcoRI sites are adjacent to each other on the vector backbone. The vector contains an XhoI site located 300 bp upstream of the BamHI site. An internal XhoI site is present in the gene sequence as shown in the figure. The resultant recombinant plasmid is digested with EcoRI and XhoI and analyzed through 1% agarose gel electrophoresis. Assuming complete digestion with EcoRI and XhoI, the DNA fragments (in base pairs) visible on the agarose gel will correspond to:

Question diagram

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6
2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2017
A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain of E. coli having the genotype lacIq crp+. Even in the absence of inducer IPTG, low levels of expression of the recombinant protein are seen (leaky expression). Which one of the following should be done to minimize such leaky expression?
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7
2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2017
Shown below is a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with BamHI or XhoI. The results from the digestion experiments are shown in (R).
Which one of the following explains the digestion results shown in (R)?

Question diagram

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8
2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2019
The Bt toxin gene from Bacillus thuringiensis used to generate genetically modified crops is
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9
2020 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2020
Which one of the following statements about Agrobacterium Ti plasmid is CORRECT?
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10
2021 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2021

Which of the following statement(s) is/are CORRECT about Agrobacterium tumefaciens?

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11
2022 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2022

Introduction of foreign genes into plant cells can be carried out using

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12
2023 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2023

Direct DNA transfer method(s) used for plant genetic engineering is(are)

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13
2010 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2010
The bacteria known to be naturally competent for transformation of DNA is
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14
2010 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2010
In transgenics, alterations in the sequence of nucleotide in genes are due to
P. Substitution
Q. Deletion
R. Insertion
S. Rearrangement
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15
2010 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2010
Somatic cell gene transfer is used for
P. transgenic animal production
Q. transgenic diploid cell production
R. in-vitro fertilization
S. classical breeding of farm animals
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16
2010 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Recombinant DNA technology
Biotechnology (BT) 2010
A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?
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