Molecular tools - Recombinant DNA technology and Other Tools in Biotechnology - Biotechnology Previous Year Questions

1

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2013

The first humanized monoclonal antibody approved for the treatment of breast cancers

A

Rituximab

B

Cetuximab

C

Bevacizumab

D

Herceptin

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2

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2013

Human genome sequencing project involved the construction of genomic library in

A

bacterial artificial chromosome

B

pBR322

C

bacteriophage

D

pcDNA3.1

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3

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2013

The nucleotide analogue used in DNA sequencing by chain termination method is

A

1',3'-dideoxy nucleoside triphosphate

B

2',3'-dideoxy nucleoside triphosphate

C

2',4'-dideoxy nucleoside triphosphate

D

2',5'-dideoxy nucleoside triphosphate

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4

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2013

Match the entries in Group I with the entries in Group II.

Group I	Group II
P. RNase P	1. Polyadenylation
Q. RNase H	2. Splicing
R. snRNAs	3. Ribozymes
S. CstF	4. DNA-RNA hybrids

A

P-3, Q-4, R-2, S-1

B

P-4, Q-3, R-2, S-1

C

P-3, Q-2, R-1, S-4

D

P-2, Q-4, R-1, S-3

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5

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2013

Match the entries in the Group I with the entries in Group II.

A

P-2, Q-1, R-3, S-4

B

P-2, Q-4, R-3, S-1

C

P-3, Q-4, R-2, S-1

D

P-1, Q-4, R-3, S-2

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6

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2013

A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are

A

EcoRI-2, BamHI-1, HindIII-2

B

EcoRI-1, BamHI-1, HindIII-2

C

EcoRI-3, BamHI-2, HindIII-1

D

EcoRI-2, BamHI-2, HindIII-1

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7

2014 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2014

The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be

A

once every 24 bases

B

once every 256 bases

C

once every 1024 bases

D

once every 4096 bases

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8

2014 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2014

The plasmid DNA was subjected to restriction digestion using the enzyme EcoRI and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in Group I with their identity in Group II.

Group I
P. Bands labeled as A
Q. Bands labeled as B
R. Bands labeled as C
S. Bands labeled as D

Group II
1. Nicked
2. Supercoiled
3. Concatemers
4. Linear

A

P-3, Q-1, R-2, S-4

B

P-1, Q-4, R-3, S-2

C

P-4, Q-3, R-1, S-2

D

P-4, Q-1, R-2, S-3

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9

2016 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2016

Which one of the following is NOT a therapeutic agent based on nucleic acid for the treatment of genetic disorders?

A

Antisense oligonucleotide

B

Ribozyme

C

Aptamer

D

Avidin

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10

2016 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2016

A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Column-I) with their corresponding source (Column-II).

Column-I	Column-II
P. Ubiquitin promoter	1. Agrobacterium tumefaciens
Q. Nos transcriptional terminator	2. Streptomyces hygroscopicus
R. bar selection marker gene	3. Escherichia coli
S. gus reporter gene	4. Zea mays

A

P-2, Q-1, R-3, S-4

B

P-2, Q-3, R-4, S-1

C

P-3, Q-4, R-1, S-2

D

P-4, Q-1, R-2, S-3

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11

2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2017

A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?

A

Northern blotting

B

Southern blotting

C

South western blotting

D

Western blotting

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12

2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2017

A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg²⁺. Which one of the following is missing in the reaction mixture?

A

Helicase

B

Single-stranded binding proteins

C

Primers

D

Reverse transcriptase

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13

2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2017

Which one of the following CANNOT be a recognition sequence for a Type II restriction enzyme?

A

GAATTC

B

AGCT

C

GCGGCGC

D

ATGCCT

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14

2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2017

EcoRI restriction sites on a 10kb DNA fragment are shown below.
[Image: DNA fragment with EcoRI sites at 2kb, 4kb, and 7kb]
Upon partial digestion, what are the lengths (in kb) of all the possible DNA fragments obtained?

A

2, 3, 4, 5, 6, 7, 8, and 10

B

2, 3, 4, 5, 6, and 7

C

2, 3, 4, and 7

D

2 and 3

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15

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2018

Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially?

A

Northern blotting

B

In situ hybridization

C

Southern blotting

D

Western blotting

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16

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2018

Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a 6-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________

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17

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2018

The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and [α-³²P]-dATP in a suitable reaction buffer. The product thus obtained was purified and subjected to gel electrophoresis followed by autoradiography.
The number of bands that will appear on the X-ray film is ______

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18

2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2019

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?

A

KpnI

B

DpnI

C

XhoI

D

MluI

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19

2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2019

Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg²⁺ concentration
Q. pH
R. Annealing temperature

A

P and Q only

B

P and R only

C

Q and R only

D

P, Q and R

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20

2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Molecular tools

Biotechnology (BT) 2019

Antibody-producing hybridoma cells are generated by the fusion of a

A

T cell with a myeloma cell

B

B cell with a myeloma cell

C

macrophage with a myeloma cell

D

T cell and a B cell

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