Analytical tools - Recombinant DNA technology and Other Tools in Biotechnology - Biotechnology Previous Year Questions

1

2016 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2016

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs⁺ DNA is given by the equation ρ = 1.66 + 0.098X_G+C where X_G+C denotes

A

total number of G and C

B

mole fraction of G+C

C

number of GC repeats

D

ratio of G+C to A+T content

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2

2016 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2016

Which one of the following techniques relies on the spin angular momentum of a photon?

A

CD spectroscopy

B

Fluorescence spectroscopy

C

IR spectroscopy

D

Raman spectroscopy

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3

2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2017

Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution?

A

Raman spectroscopy

B

IR spectroscopy

C

UV spectroscopy

D

NMR spectroscopy

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4

2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2017

Enzyme-linked immunosorbent assay (ELISA) is used for

A

quantifying antibody levels in blood

B

determining the molecular weight of an antigen

C

purifying proteins from biological fluids

D

determining the molecular weight of an antibody

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5

2017 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2017

The circular dichroism spectra of three proteins P, Q, and R are given below:
Choose the correct combination.

A

P: α-helix, Q: β-sheet, and R: Random coil

B

P: β-sheet, Q: α-helix, and R: Random coil

C

P: α-helix, Q: Random coil, and R: β-sheet

D

P: Random coil, Q: α-helix, and R: β-sheet

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6

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2018

An analysis of DNA-protein interactions was carried out using all DNA-protein complexes in the protein data bank (PDB). The frequency distribution of four amino acid residues, represented as P, Q, R and S, occurring in non-covalent interactions between the protein and DNA backbone is shown below.
Which one of the following is correct?

A

P-Lys, Q-Arg, R-Gln, S-Glu

B

P-Gln, Q-Glu, R-Lys, S-Arg

C

P-Asn, Q-Asp, R-Arg, S-Lys

D

P-His, Q-Glu, R-Gln, S-Lys

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7

2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2019

Group I lists spectroscopic methods and Group II lists biomolecular applications of these methods. Match the methods in Group I with the applications in Group II.

Group I	Group II
P. Infrared	1. Identification of functional groups
Q. Circular Dichroism	2. Determination of secondary structure
R. Nuclear Magnetic Resonance	3. Estimation of molecular weight
	4. Determination of 3-D structure

A

P-4, Q-3, R-1

B

P-2, Q-1, R-3

C

P-1, Q-2, R-4

D

P-3, Q-2, R-4

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8

2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2019

Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?

A

Polymerase chain reaction

B

DNA microarray

C

Northern hybridization

D

Southern hybridization

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9

2021 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2021

Which of the following nucleus/nuclei is/are NMR active?

A

¹H

B

¹³C

C

¹⁶O

D

³²S

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10

2022 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2022

Match the stationary phase (Column I) with its corresponding chromatography technique (Column II).

Column I	Column II
P. Protein A	1. Size exclusion chromatography
Q. Sephadex	2. Ion-exchange chromatography
R. Phenylsepharose	3. Affinity chromatography
S. Diethylaminoethyl cellulose	4. Hydrophobic interaction chromatography

A

P-1, Q-4, R-2, S-3

B

P-3, Q-1, R-4, S-2

C

P-3, Q-4, R-2, S-1

D

P-4, Q-1, R-3, S-2

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11

2022 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2022

Which of the following spectroscopic technique(s) can be used to identify all the functional groups of an antibiotic contaminant in food?
P. Infrared
Q. Circular dichroism
R. Nuclear magnetic resonance
S. UV-Visible

A

P only

B

P and R only

C

P, Q and R only

D

P, Q, R and S

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12

2023 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2023

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase

A

P and R

B

P and Q

C

Q and S

D

R and S

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13

2023 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2023

Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?

A

Circular dichroism spectroscopy

B

Fourier transform infrared spectroscopy

C

Mass spectrometry

D

X-ray crystallography

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14

2024 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2024

In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by ________.

A

covalent bonds

B

electrostatic interactions

C

ionic bonds

D

van der Waals forces

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15

2024 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2024

Match the item in Column I with the corresponding technique in Column II.

Column I	Column II
P. Blue laser	1. Electron microscopy
Q. Tungsten filament	2. Fluorescence activated cell sorting
R. \( ^{15}N \) labelled protein	3. Electrophoresis
S. Polyacrylamide	4. Nuclear magnetic resonance spectroscopy

A

P-2; Q-3; R-1; S-4

B

P-2; Q-1; R-4; S-3

C

P-3; Q-1; R-4; S-2

D

P-1; Q-2; R-4; S-3

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16

2025 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2025

An octapeptide composed of these L-amino acids – Lys, Thr, Ser, Met, Arg, Trp, Tyr, Glu – was subjected to analyses by the following outcomes:
P. The N-terminal sequencing analysis by Sanger’s method yielded ‘Ser’ at the N-terminus
Q. Chymotrypsin treatment gave a pentapeptide, a ‘Tyr’ containing dipeptide and a free ‘Glu’
R. Cyanogen bromide treatment gave two tetrapeptides
S. Trypsin treatment gave two tripeptides and a dipeptide
Which one of the following is the correct octapeptide sequence?

A

Ser-Tyr-Arg-Met-Lys-Thr-Trp-Glu

B

Ser-Arg-Lys-Met-Tyr-Thr-Trp-Glu

C

Ser-Met-Lys-Arg-Thr-Tyr-Trp-Glu

D

Ser-Arg-Met-Lys-Trp-Thr-Tyr-Glu

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17

2025 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2025

Which of the following methods is/are used for identifying histone modifications?

A

ChIP-seq

B

Mass spectrometry

C

Immunofluorescence

D

Patch-clamp electrophysiology

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18

2010 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2010

Match Group I with Group II

Group I	Group II
P. Real Time-PCR	1. Biochips
Q. 2-D Electrophoresis	2. Syber Green
R. Affinity chromatography	3. Antibody linked sephrose beads
S. Microarray	4. Ampholytes

A

P-1, Q-2, R-4, S-3

B

P-2, Q-3, R-4, S-1

C

P-2, Q-4, R-3, S-1

D

P-3, Q-2, R-1, S-4

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19

2011 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2011

Match the items in Group I with Group II.
Group I
P. Circular dichroism
Q. X-ray crystallography
R. Freeze-drying
S. Ultracentrifugation

Group II
1. Concentration
2. Sedimentation coefficient
3. Secondary structure determination
4. Tertiary structure determination

A

P-4, Q-1, R-2, S-3

B

P-1, Q-4, R-3, S-2

C

P-2, Q-3, R-4, S-1

D

P-3, Q-4, R-1, S-2

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20

2012 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Analytical tools

Biotechnology (BT) 2012

In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with

A

ferrous ammonium sulfate

B

uranium acetate

C

sodium chloride

D

basic fuchsin

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