LIFE SCIENCE PART C (JUNE 2016) 2016

The standard free energy change (ΔG0) per mole for the reaction at in an open system is –1000 cal/mole. What is the approximate free energy change (ΔG) when the concentration of A and B and are 100 micromolar and 100 millimolar, respectively?

Indicate which one of the following statements about nucleic acids and protein structures is correct.

In a mitochondrial respiration experiment, a researcher observed the following profile of oxygen consumption upon addition of following compounds at times I, II and III.(a) ADP + Pi(b) Dinitrophenol, an uncoupler(c) Oligomycin, an ATPase inhibitor (d)Cyanide(e) SuccinateWhich of the following describes the profile appropriately?

A researcher has developed a program to evaluate the stability of a protein by substituting each amino acid at a time by the other 19 amino acids. For a protein, a researcher has observed the following changes in stability upon substitution of amino acids in loops, helices, sheets, protein core and on the protein surface.
Substitutions in
(a) loops are more tolerant
(b) sheets are more tolerant
(c) core is less tolerant
(d) helices are less tolerant
(e) surface is more tolerant
Which of the above statements are correct?

Indicate the names of the following molecules

The turnover number and specific activity of an enzyme (molecular weight 40,000 D) in a reaction (Vmax= 4μmol of substrate reacted/ min, enzyme amount = 2μg) are