Plant Tissue Culture and Regeneration - Plant Breeding, Genetic Modification, Genome Editing - Life Sciences Previous Year Questions

1

2007 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2007

Gynogenesis is a process of development of haploid plants

A

from a fertilized cell of female gametophyte

B

from an unfertilized cell of female gametophyte

C

from isolated pollen grains

D

by selective elimination of chromosomes following distant hybridization

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2

2007 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2007

Which of the following factors is critical for haploidy induction?

A

Presence of optimum levels of auxin and cytokinin in the medium

B

Treatment of donor plants with phytohormones

C

Use of colchicine in the medium

D

Induction and proliferation of callus from anther culture

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3

2007 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2007

Identify the correct statements.
P: Xylogenesis is defined as the differentiation of parenchyma into specialized xylem cell
Q: First anther culture was reported by Guha and Maheshwari
R: Totipotency was reported by Sundarland
S: In vitro fertilization reported by Hofmeister

A

P, S

B

P, Q

C

P, R

D

R, S

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4

2007 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2007

Encapsulated somatic embryo in alginate beads produce artificial seeds. Identify the correct statements.
P: Artificial seed is a genetically modified agricultural product
Q: Artificial seed is a patented product for pharmaceutical industry
R: Artificial seeds can be stored and transferred to soil for germination
S: Somatic embryo of single cell origin produce genetically uniform plants

A

P, S

B

P, Q

C

Q, R

D

R, S

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5

2008 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2008

Which one of the following is the growth factor used for growth of tissues and organs in plant tissue culture?

A

Cysteine

B

Cytokinin

C

Cytidylate

D

Cyclic AMP

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6

2008 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2008

Multiplication of genetically identical copies of a cultivar by asexual reproduction is known as

A

aclonal propagation

B

vegetative propagation

C

polyclonal propagation

D

clonal propagation

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7

2008 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2008

Determine the correctness or otherwise of following Assertion [a] and Reason [r]
Assertion: The enzymatic degradation of cell wall to obtain single cell called protoplast has helped immensely in developing somatic cell genetics in plants
Reason: In plants or animals, fusion of two cells must occur through the plasma membrane

A

Both [a] and [r] are true and [r] is the correct reason for [a]

B

Both [a] and [r] are true but [r] is not the correct reason for [a]

C

[a] is true but [r] is false

D

[a] is false but [r] is true

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8

2008 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2008

Some living cells (e.g. plant cell) have the capacity to give rise to whole organism. The term used to describe this property is

A

morphogenesis

B

androgenesis

C

totipotency

D

organogenesis

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9

2009 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2009

To produce plants that are homozygous for all traits, the best choice is

A

Protoplast culture

B

Cell suspension culture

C

Anther and pollen culture

D

Apical meristem culture

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10

2011 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2011

The transition of water molecule from liquid to glassy state during cryopreservation is termed as

A

Vitrification

B

Hyperhydricity

C

Cryoprotectant

D

Habituation

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11

2011 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2011

A photoautotrophic micropropagation system can be established by increasing the

A

sucrose concentration in the culture medium

B

CO₂ concentration in the culture medium

C

agar concentration in the culture medium

D

NH₄⁺ concentration in the culture medium

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12

2012 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2012

Which of the following statements are CORRECT for somatic cell hybridization?
P. For fusion of protoplast, dimethylsulfoxide (DMSO) is used as a fusogen
Q. The enzyme ‘Cellulase Onozuka’ used for protoplast isolation is sourced from Trichoderma viride
R. The first report of somatic hybrid plants resulted from the fusion of protoplasts of Nicotiana glauca and N. tabacum
S. Viability of isolated protoplasts can be determined by Evan’s blue staining

A

P, Q

B

Q, S

C

R, S

D

Q, R

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13

2013 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2013

The two enzymes commonly used for isolation of protoplasts from plants are

A

Cellulase and Lipase

B

Cellulase and Amylase

C

Pectinase and Cellulase

D

Pectinase and Lipase

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14

2015 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2015

Statements given below are either TRUE (T) or FALSE (F). Find the correct combination.
P. Somatic embryo is unipolar in nature
Q. Heterokaryon can be selected using a fluorescence-activated cell sorter (FACS)
R. The term somaclonal variation is coined by Larkin and Scowcroft
S. Differentiation of shoot buds during in vitro culture is known as somatic embryogenesis

A

P-T, Q-F, R-T, S-F

B

P-F, Q-T, R-F, S-T

C

P-T, Q-F, R-F, S-T

D

P-F, Q-T, R-T, S-F

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15

2015 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2015

Double haploids in plants can be induced by

A

Mitomycin-C

B

Mirn

C

Colchicine

D

5-Azacytidine

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16

2019 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2019

Cybrids may arise through the

A

fusion of a normal protoplast with another nucleated protoplast of different origin

B

fusion between a normal protoplast and a protoplast containing viable nucleus

C

elimination of one of the nuclei from heterokaryon formed from two protoplasts of different origin

D

fusion of a normal cell with another nucleated cell

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17

2019 · Life Sciences · Plant Breeding, Genetic Modification, Genome Editing · Plant Tissue Culture and Regeneration

Life Sciences (XL) 2019

Which one of the following methods is INCORRECT with respect to haploid plant production? It can be produced

A

from an unfertilized egg cell

B

from nucellar tissue

C

from isolated pollen culture

D

by distant hybridization, followed by selective elimination of chromosomes of one of the parents

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