Mutation, DNA Repair and Gene Transfer - Microbial Genetics - Life Sciences Previous Year Questions

1

2007 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2007

An Hfr bacterium is one that contains

A

Many unusual plasmids

B

Chromosomal material acquired from a recipient cell

C

The ability to undergo transduction

D

A plasmid integrated into its chromosome

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2

2009 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2009

A tryptophan auxotrophic Hfr strain of E. coli is mixed with F^– histidine auxotroph and plated on to minimal medium to select for prototrophs. The prototrophs are formed due to

A

Conjugation

B

Transduction

C

Transformation

D

Transfection

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3

2009 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2009

The high frequency of recombinants obtained from a cross between Hfr and F^– strains is because

A

the F factor integrated into the chromosome mediates transfer of large regions of chromosome into the recipient.

B

the strain bears high number of F pili on its surface.

C

there are high number of copies of the F plasmid per cell.

D

it induces recombination at high rate.

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4

2010 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2010

Ames test is performed to detect

A

mutagen

B

pH

C

nutrient stress

D

salinity

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5

2011 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2011

To select for spontaneously arising histidine auxotrophs in a population, you would use a medium containing

A

Histidine and penicillin

B

Penicillin but no histidine

C

Histidine and lysozyme

D

Lysozyme but no histidine

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6

2011 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2011

In which one of the following organisms, it is easiest to distinguish mutations on adjacent base pairs of DNA through genetic recombination experiments?

A

Bacteriophages

B

Yeast

C

Escherichia coli

D

Bacillus subtilis

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7

2013 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2013

Retroviruses, like the influenza virus, escape the detection of pre-existing antibodies in the host by generating surface antigen variants. They do so:

A

By editing the surface antigen post-translationally

B

Because RNA polymerase of these viruses display high mutation rate during RNA synthesis

C

Because the surface antigens are attacked by the proteases present in the host cell

D

Because DNA polymerase of the host mutates the viral genome in the infected cell

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8

2014 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2014

Which of the following statements are TRUE regarding recA mutants of E. coli?

(P) Exhibit much reduced recombination
(Q) Do not survive UV irradiation
(R) Show no effect on doubling time
(S) Exhibit pleiotropy

A

P, Q & R only

B

P, Q & S only

C

P, R & S only

D

Q, R & S only

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9

2014 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2014

Which one of the following is NOT involved in horizontal gene transfer?

A

Conjugation

B

Transformation

C

Transduction

D

Mutation

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10

2019 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2019

Which of the following processes are involved in horizontal gene transfer (HGT)? (i) Conjugation (ii) Transduction (iii) Binary fission (iv) Transformation (v) Translesion synthesis (vi) Transversion

A

(i), (iii), (iv)

B

(i), (iv), (vi)

C

(ii), (iv), (v)

D

(i), (ii), (iv)

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11

2023 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2023

Which one of the following conjugations will result in formation of merodiploids?

A

F⁺ donor × F⁻ recipient

B

Hfr donor × F⁻ recipient

C

F⁻ donor × F⁻ recipient

D

F⁺ donor × Hfr recipient

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12

2023 · Life Sciences · Microbial Genetics · Mutation, DNA Repair and Gene Transfer

Life Sciences (XL) 2023

The spontaneous, and induced mutations in bacteria can be distinguished by

A

fluctuation test

B

replica plating

C

disc diffusion test

D

use-dilution test

Check answer

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