Immunological Techniques and Flow Cytometry - Analytical Techniques - Life Sciences Previous Year Questions

1

2007 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2007

Match the techniques mentioned in Column A with their applications given in Column B.

A	B
P. PCR	1. Identification of transcription factor binding sites in chromatin
Q. DNA microarray	2. Identification of HIV infected patients using serum samples
R. ELISA	3. Isolation of mouse homologue of a yeast gene
	4. Analysis of differential gene expression in cancer and normal cells

A

P-4, Q-1, R-3

B

P-3, Q-4, R-2

C

P-4, Q-1, R-2

D

P-3, Q-2, R-1

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2

2007 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2007

Match the methods available on world wide web in group 1 for performing the jobs listed in group 2

A

P-1, Q-3, R-2, S-4

B

P-2, Q-3, R-2, S-4

C

P-3, Q-4, R-1, S-4

D

P-3, Q-2, R-1, S-4

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3

2007 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2007

The antigen is expressed as transmembrane protein with a single epitope on its extracellular domain. The cells that survived (assume 100% transfection and expression of protein) were incubated with a radio labeled Fab fragment (specific activity: 100 cpm/picomole) against this epitope. After washing, the cell pellet has 1000 cpm. The average number of epitopes present on a single recombinant alga are

A

6 X 10⁹

B

1 X 10⁵

C

6 X 10³

D

1 X 10⁶

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4

2008 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2008

In hybridoma technology, the myeloma cells used

A

lack HGPRTase

B

lack the ability to produce Ig

C

lack both HGPRTase and ability to produce Ig

D

lack thymidine kinase

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5

2008 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2008

In a fluorescein labeled antibody to μ heavy chain and rhodamine labeled antibody to δ heavy chain, the fluorescent antibody staining pattern of the progenitor B cells ( Pro-B cells) will be

A

anti-μ staining in cytoplasm and on membrane

B

anti-μ and anti-δ staining in cytoplasm and on membrane

C

no cytoplasmic or membrane staining with either anti μ or δ antibody

D

anti-δ staining on the membrane

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6

2009 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2009

Cells expressing Epidermal Growth Factor (EGF) receptors were treated (T) or untreated (U) with EGF. The cells were lysed and immunoprecipitated with EGF receptor-specific antibodies. The immunoprecipitate was analyzed by Western blotting with antibodies specific to
i) phosphotyrosine ii) phosphothreonine iii) tyrosine kinases iv) threonine kinases.
Which of these antibodies would detect a band under T and U conditions ?

A

T – i & ii; U – iii

B

T – ii & iv; U – iv

C

T – i & ii; U – i

D

T – i & iii; U – ii

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7

2009 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2009

Which of the following are commonly used as reporter genes ?
P. NPT gene
Q. Luciferase gene
R. CFTR gene
S. GFP gene

A

Q, S

B

R, S

C

P, R

D

P, Q

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8

2009 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2009

Match the items in Group 1 with correct options in Group 2 :

Group 1	Group 2
P. DNA footprinting	1. Protein-protein interaction
Q. Yeast two-hybrid system	2. VNTR
R. DNA fingerprinting	3. DNA binding protein
S. SAGE	4. Transcriptome analysis

A

P-1, Q-2, R-4, S-3

B

P-3, Q-1, R-2, S-4

C

P-3, Q-4, R-1, S-2

D

P-4, Q-2, R-1, S-3

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9

2011 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2011

Horseradish peroxidase and alkaline phosphatase are the two enzymes commonly utilized as reagents in ELISA, because these enzymes

A

are colored proteins

B

are very small

C

have high turnover number

D

bind to ELISA plates

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10

2011 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2011

The DNA content of a nucleus can be measured by

A

ESR Spectroscopy

B

FTIR Spectroscopy

C

Flow Cytometry

D

X-Ray Crystallography

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11

2012 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2012

Select the technique most appropriate to demonstrate that lactose induces the synthesis of β-galactosidase enzyme.

A

Northern Blot

B

Western Blot

C

Quantitative PCR

D

Southern Blot

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12

2014 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2014

10% of the population in a town is HIV⁺. A new diagnostic kit for HIV detection is available; this kit correctly identifies HIV⁺ individuals 95% of the time, and HIV⁻ individuals 89% of the time. A particular patient is tested using this kit and is found to be positive. The probability that the individual is actually positive is ______

Check answer

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13

2014 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2014

The affinity of an antibody can be determined quantitatively by

A

MALDI-TOF MS

B

Isoelectric focusing

C

SDS-PAGE

D

Equilibrium dialysis

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14

2015 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2015

Limulus Amoebocyte Lysate (LAL) assay is used to identify the presence of

A

endotoxin

B

exotoxin

C

anthrax toxin

D

tetanus toxin

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15

2016 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2016

An N-terminal His-tagged protein of molecular weight 40 kDa was purified using Ni-NTA column. This protein sample was subjected to SDS-PAGE. A western blot of the same using anti-His antibodies is shown below.
Which one of the following interpretations is correct?

A

Only the His-tag of the protein got removed

B

The protein forms oligomers

C

The purified protein sample is homogeneous

D

The protein has a stable N-terminal 20 kDa domain

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16

2017 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2017

Which one of the following features is NOT appropriate for Fab fragment of IgG ?

A

Contains antigen binding site

B

Contains an intact L chain

C

Two fragments are formed from one IgG molecule

D

Mediates complement fixation in the intact IgG molecule

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17

2018 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2018

Which one of the following properties of the myeloma cells is used in the hybridoma technology to generate monoclonal antibody?

A

lack of thymidylate synthase

B

over-expression of hypoxanthine-guanine phosphoribosyl transferase

C

over-expression of inosine 5'-monophosphate cyclohydrolase

D

lack of hypoxanthine-guanine phosphoribosyl transferase

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18

2020 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2020

Binding of an antibody to its cognate antigen does NOT involve

A

Covalent bonds

B

Electrostatic forces

C

Van der Waals forces

D

Hydrogen bonds

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19

2020 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2020

Which ONE of the following techniques can be used for detecting the subcellular localization of serotonin receptor in intact cells?

A

Immunoelectron microscopy

B

SDS-PAGE

C

Fluorescence in-situ hybridization

D

Differential centrifugation

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20

2023 · Life Sciences · Analytical Techniques · Immunological Techniques and Flow Cytometry

Life Sciences (XL) 2023

Match the tasks given in Group I with the associated techniques conventionally used as listed in Group II.

A

P-2; Q-1; R-3; S-4; T-5

B

P-3; Q-4; R-1; S-2; T-5

C

P-5; Q-4; R-1; S-2; T-3

D

P-3; Q-5; R-1; S-2; T-4

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