Computational tools - Recombinant DNA technology and Other Tools in Biotechnology - Biotechnology Previous Year Questions

1

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2013

The suitable substitution matrix to align closely related sequences is

A

PAM 250 or BLOSUM 80

B

PAM 40 or BLOSUM 80

C

PAM 120 or BLOSUM 40

D

PAM 250 or BLOSUM 40

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2

2013 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2013

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: UPGMA method produces ultrametric tree.
Reason: Sequence alignment is converted into evolutionary distances in UPGMA method.

A

Both (a) and (r) are true and (r) is the correct reason for (a)

B

Both (a) and (r) are true and (r) is not the correct reason for (a)

C

is true but (r) is false

D

is false but (r) is true

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3

2014 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2014

The algorithm for BLAST is based on

A

Dynamic Programming

B

Hidden Markov Model

C

k-tuple analysis

D

Neural Network

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4

2014 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2014

In an affine gap penalty model, if the gap opening penalty is -20, gap extension penalty is -4 and gap length is 8, the gap score is ______.

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5

2014 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2014

The amino acid substitution matrices in decreasing order of stringency for comparing protein sequences are

A

PAM250, PAM120, PAM100

B

PAM100, PAM120, PAM250

C

PAM250, PAM100, PAM120

D

PAM120, PAM250, PAM100

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6

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2018

Which of the following components constitute a molecular mechanics force field?
P. Bond stretching
Q. Bond angle bending
R. Torsional bond rotation
S. Non-bonded interactions

A

P and Q only

B

P, Q and R only

C

P, Q and S only

D

P, Q, R and S

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7

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2018

Which one of the following BLAST search programs is used to identify homologs of a genomic DNA query in a protein sequence database?

A

blastp

B

blastn

C

blastx

D

tblastn

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8

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2018

Identify the character based method(s) used for the construction of a phylogenetic tree.
P. Maximum parsimony
Q. Neighbor joining
R. Maximum likelihood
S. Bootstrapping

A

Q only

B

P and R only

C

Q and S only

D

S only

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9

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2018

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion: Ab initio gene finding algorithms that predict protein coding genes in eukaryotic genomes are not completely accurate.
Reason: Eukaryotic splice sites are difficult to predict.

A

Both [a] and [r] are false

B

[a] is true but [r] is false

C

Both [a] and [r] are true and [r] is the correct reason for [a]

D

Both [a] and [r] are true but [r] is not the correct reason for [a]

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10

2018 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2018

Consider the following alignment of two DNA sequences:
AGT AAC
AA – AC
Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment is (up to one decimal place) ______

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11

2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2019

Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm?
P. A larger E-value indicates higher sequence similarity
Q. E-value < 10^-10 indicates sequence homology
R. A higher BLAST score indicates higher sequence similarity
S. E-value > 10¹⁰ indicates sequence homology

A

P, Q and R only

B

Q and R only

C

P, R and S only

D

P and S only

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12

2019 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2019

Which one of the following is a database of protein sequence motifs?

A

PROSITE

B

TrEMBL

C

SWISSPROT

D

PDB

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13

2021 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2021

Which one of the following methods is used to test the significance of a predicted phylogeny?

A

Bootstrap

B

Maximum likelihood

C

Maximum parsimony

D

Minimum evolution

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14

2021 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2021

Which one of the following tools is used to compare all the possible six-open reading frames of a given nucleotide query sequence with all the available six-open reading frames of the nucleotide sequence database?

A

BLASTN

B

BLASTX

C

TBLASTN

D

TBLASTX

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15

2022 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2022

Which of the following is NOT used for generating an optimal alignment of two nucleotide sequences?

A

Gap penalties

B

Match scores

C

Mismatch scores

D

Nucleotide composition

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16

2023 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2023

Which one of the following programs is used for finding distantly related (or remote) protein homologs?

A

BLASTN

B

BLASTX

C

PSI-BLAST

D

TBLASTX

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17

2023 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2023

Which one of the following is used for global alignment of two protein sequences?

A

Chou-Fasman method

B

Garnier-Osguthorpe-Robson (GOR) method

C

Needleman-Wunsch algorithm

D

Smith-Waterman algorithm

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18

2025 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2025

Correctly match the following Bioinformatic tool/Database with its respective Utility.

Bioinformatic tool/Database	Utility
P. BLAST	1. Database for 3D protein structures
Q. Bowtie	2. Tool to identify similarity of a query sequence to existing sequences available in databanks
R. AlphaFold	3. Tool to align short read DNA sequences obtained from Next-generation sequencing to a reference genome
S. PDB	4. AI tool to predict protein structures

A

P-2; Q-3; R-1; S-4

B

P-2; Q-3; R-4; S-1

C

P-3; Q-2; R-4; S-1

D

P-4; Q-1; R-2; S-3

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19

2010 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2010

Program used for essentially local similarity search is

A

BLAST

B

RasMol

C

ExPASY

D

SWISS-PROT

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20

2010 · Biotechnology · Recombinant DNA technology and Other Tools in Biotechnology · Computational tools

Biotechnology (BT) 2010

Accession number is a unique identification assigned to a

A

single database entry for DNA/Protein

B

single database entry for DNA only

C

single database entry for Protein only

D

multiple database entry for DNA/Protein

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