Molecular Biology - Genetics, Cellular and Molecular Biology - Biotechnology Previous Year Questions

1

2013 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2013

Under alkaline conditions, DNA is more stable than RNA because

A

RNA forms secondary structures

B

RNA is a single stranded molecule

C

RNA has uracil in place of thymidine

D

RNA is susceptible to hydrolysis

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2

2013 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2013

Which one of the following modifications is common to both protein and DNA?

A

SUMOylation

B

Nitrosylation

C

Methylation

D

Ubiquitination

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3

2013 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2013

The RNA primer synthesized during the replication process in bacteria is removed by

A

DNA gyrase

B

primase

C

DNA polymerase I

D

DNA polymerase II

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4

2013 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2013

Calculate the percentage sequence identity for the pairwise alignment given below. ________
H E L L O –
Y E L L O W

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5

2014 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2014

The 4-amino or 4-keto group of pyrimidine bases is located in the

A

major groove of the double stranded DNA

B

minor groove of the double stranded DNA

C

minor groove of the B form DNA but not the A form DNA

D

major groove of the B form DNA but not the A form DNA

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6

2014 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2014

In a mouse genome, the numbers of functional Vα, Jα, Vβ, Dβ, Jβ gene segments are 79, 38, 21, 2 and 11, respectively. The total number of possible combinations for αβ T cell receptors are ____ ×10⁶.

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7

2014 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2014

For their efficient translation, eubacterial mRNAs possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence (ASD) in the ribosomes. The correct statement is

A

ASD is present in 5S rRNA

B

ASD is present in 23S rRNA

C

ASD is present in 16S rRNA

D

ASD is formed by the interaction of the 16S rRNA with the 23S rRNA upon docking of the 50S subunit on the 30S subunit of the ribosomes

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8

2014 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2014

The length of the minimum unique stretch of DNA sequence that can be found only once in a 3 billion base pairs long genome is

A

14

B

15

C

16

D

18

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9

2014 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2014

Which of the following statements with respect to the orientation of the nitrogenous bases to the pentose sugars, and the puckering of the sugar, is correct?

A

Anti, and 2’-endo in A form DNA

B

Anti, and 2’-endo in B form DNA

C

Syn, and 3’-endo in A form DNA

D

Syn, and 3’-endo in B form DNA

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10

2014 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2014

The active site in the alpha/beta barrel structures is usually located

A

inside the barrel

B

at the amino side of the strands

C

at the carboxy side of the strands

D

at any arbitrary site

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11

2016 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2016

What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?

A

Only the repressor remains bound to the operator

B

Only the cyclic AMP-Catabolic Activator Protein (cAMP-CAP) complex remains bound to the CAP binding site

C

Neither the repressor nor cAMP-CAP complex remain bound to their respective binding sites

D

Both the repressor and cAMP-CAP complex remain bound to their respective binding sites

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12

2016 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2016

Junctional diversity of antibody molecules results from

A

the addition of switch region nucleotides

B

the addition of N and P nucleotides

C

the joining of V, D and J segments

D

mutations in complementarity-determining regions

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13

2016 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2016

Based on their function, find the ODD one out.

A

miRNA

B

siRNA

C

shRNA

D

snRNA

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14

2016 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2016

Which set of the following events occurs during the elongation step of translation?
P. Attachment of mRNA with the smaller subunit of ribosome
Q. Loading of correct aminoacyl-tRNA into the A site
R. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P site
S. Dissociation of the ribosomal subunits
T. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome

A

P, Q and R

B

P, Q and T

C

Q, R and T

D

R, S and T

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15

2016 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2016

A DNA sequence, 5'-ATGGACGTGCTTCCCAAAAGCATCGGGC-3', is mutated to obtain
P. 5' - ATGGACGTGCTTCaCAAAAGCATCGGGC- 3'
Q. 5' - ATGGACGTGCTTCCCgAAAGCATCGGGC- 3'
R. 5' - ATGGACGTGCTTCC -AAAGCATCGGGC- 3'
S. 5' - ATGGACGTGCTTCCCAAaGCATCGGGC- 3'
T. 5' - ATGGACgAGCTTCCCAAAGCATCGGGC- 3'
[Point mutations are shown in the lower case or ‘-’ within the sequences]
Which of the above mutant sequences DO NOT have frame-shift?

A

P, Q and S

B

P, S and T

C

Q, R and S

D

Q, S and T

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16

2017 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2017

If the nucleotide composition (%) of a viral genome is A = 10, U = 20, C = 40, and G = 30, which one of the following is this genome?

A

Double stranded RNA

B

Single stranded RNA

C

Single stranded DNA

D

Double stranded DNA

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17

2017 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2017

If a protein contains four cysteine residues, the number of different ways they can simultaneously form two intra-molecular disulphide bonds is ______.

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18

2017 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2017

During protein synthesis, tRNAs are NOT involved in

A

charging

B

initiation

C

elongation

D

termination

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19

2017 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2017

The transcription factor X binds a 10 base pair DNA stretch. In the DNA of an organism, X was found to bind at 20 distinct sites. An analysis of these 20 binding sites showed the following distribution:

Base	Position in the binding site
	1	2	3	4	5	6	7	8	9	10
A	11	0	0	0	16	2	4	0	4	3
T	3	0	19	0	1	3	4	20	2	4
G	4	20	0	0	2	4	6	0	12	2
C	2	0	1	20	1	11	6	0	2	11

What is the consensus sequence for the binding site of X?

A

NGTCNNNTNN

B

AGTCACNTGC

C

CACCTANCTG

D

ANNACGNNGC

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20

2018 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2018

The repeat sequence of telomere in humans is

A

5'-TATAAT-3'

B

5'-TTAGGG-3'

C

5'-GGGCCC-3'

D

5'-AAAAAAA-3'

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