Microbiology - General Biology - Biotechnology Previous Year Questions

1

2013 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2013

Phylum proteobacteria is subdivided into α-, β-, γ-, δ- and ε-proteobacteria based on

A

G+C content

B

23S rRNA sequences

C

tRNA sequences

D

16S rRNA sequences

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2

2013 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2013

Match the cell structures in Group I with the organisms in Group II.

Group I	Group II
P. Endospores	1. Methanobacterium
Q. Bipolar flagella	2. Treponema
R. Pseudomurine in cell wall	3. Spirillum
S. Periplasmic flagella	4. Clostridium

A

P-4, Q-3, R-1, S-2

B

P-4, Q-3, R-2, S-1

C

P-3, Q-4, R-1, S-2

D

P-4, Q-1, R-3, S-2

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3

2013 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2013

Match the antibiotics in Group I with the targets in Group II.

Group I	Group II
P. Sulfonamide	1. Peptidoglycan synthesis
Q. Quinolones	2. Peptide chain elongation
R. Erythromycin	3. Folic acid biosynthesis
S. Cephalosporin	4. Topoisomerase

A

P-3, Q-4, R-1, S-2

B

P-2, Q-4, R-3, S-1

C

P-4, Q-1, R-2, S-3

D

P-3, Q-4, R-2, S-1

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4

2014 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2014

Gram-positive bacteria are generally resistant to complement-mediated lysis because

A

thick peptidoglycan layer prevents insertion of membrane attack complex into the inner membrane

B

Gram-positive bacteria import the membrane attack complex and inactivate it

C

membrane attack complex is degraded by the proteases produced by the Gram-positive bacteria

D

Gram-positive bacteria cannot activate the complement pathway

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5

2014 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2014

A bacterium belonging to cocci group has a diameter of 2 μm. The numerical value of the ratio of its surface area to volume (in μm⁻¹) is ____

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6

2016 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2016

Bacteria with two or more flagella at one or both ends are called

A

amphitrichous

B

peritrichous

C

lophotrichous

D

atrichous

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7

2016 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2016

Which family of viruses has single stranded DNA?

A

Herpesviridae

B

Poxviridae

C

Retroviridae

D

Parvoviridae

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8

2016 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2016

Which of the following are TRUE for Treponema pallidum?
P. It is the causative agent of syphilis
Q. It is a spirochete
R. It is a non-motile bacterium
S. It is generally susceptible to penicillin
Choose the correct combination.

A

P, Q and R only

B

P, Q and S only

C

P, R and S only

D

Q, R and S only

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9

2016 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2016

Which combination of the following statements is CORRECT for cyanobacteria?
P. They can perform oxygenic photosynthesis
Q. Usually filamentous forms are involved in nitrogen fixation
R. Nitrogen fixation occurs in heterocysts
S. They cannot grow in a mineral medium exposed to light and air

A

P, Q and R

B

P, S and R

C

Q, R and S

D

P, Q and S

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10

2016 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2016

Which of the following events occur during the stationary phase of bacterial growth?
P. Rise in cell number stops
Q. Spore formation in some Gram-positive bacteria such as Bacillus subtilis
R. Cell size increases in some Gram-negative bacteria such as Escherichia coli
S. Growth rate of bacterial cells nearly equals their death rate
T. Decrease in peptidoglycan crosslinking

A

P, Q and S only

B

P, S and T only

C

Q, R and S only

D

P, R and T only

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11

2017 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2017

Which one of the following organisms is an indicator of fecal contamination?

A

Escherichia coli

B

Streptococcus lactis

C

Bacillus subtilis

D

Lactobacillus acidophilus

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12

2017 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2017

Assertion [a]: Gram negative bacteria show staining with saffranin.
Reason [r]: Gram negative bacteria have an outer membrane with lipopolysaccharides.

A

Both [a] and [r] are true and [r] is the correct reason for [a].

B

Both [a] and [r] are true but [r] is not the correct reason for [a].

C

Both [a] and [r] are false.

D

[a] is true but [r] is false.

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13

2017 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2017

Match the organisms in Column I with the characteristics in Column II

Column I	Column II
P. Methanococcus	1. Halophile
Q. Dunaliella	2. Acidophile
R. Sulfolobus	3. Mesophile
S. Escherichia	4. Barophile

A

P-4, Q-3, R-2, S-1

B

P-3, Q-2, R-4, S-1

C

P-2, Q-1, R-4, S-3

D

P-4, Q-1, R-2, S-3

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14

2018 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2018

Which one of the following is an example of a neurotoxin?

A

Cholera toxin

B

Streptolysin-O

C

Botulinum toxin

D

Diphtheria toxin

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15

2018 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2018

Which of the following are true about bacterial superoxide dismutase?
P. Present in obligate aerobes
Q. Present in facultative anaerobes
R. Present in aerotolerant anaerobes
S. Absent in obligate aerobes

A

P and Q only

B

P, Q and R only

C

P and R only

D

Q and S only

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16

2018 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2018

Which of the following are true with regard to anaerobic respiration in bacteria?
P. The final electron acceptor is an inorganic substance other than molecular oxygen
Q. The number of ATP molecules produced per glucose molecule is more than that produced in aerobic respiration
R. The number of ATP molecules produced per glucose molecule is less than that produced in aerobic respiration
S. Only substrate level phosphorylation is used to generate ATP

A

P and S only

B

Q and S only

C

P and R only

D

P, Q and S only

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17

2019 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2019

Match the human diseases in Group I with the causative agents in Group II.

Group I	Group II
P. Amoebiasis	1. Leishmania donovani
Q. African sleeping sickness	2. Trypanosoma cruzi
R. Kala azar	3. Entamoeba histolytica
S. Chagas’ disease	4. Trypanosoma gambiense

A

P-3, Q-4, R-2, S-1

B

P-3, Q-2, R-1, S-4

C

P-3, Q-4, R-1, S-2

D

P-4, Q-3, R-1, S-2

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18

2019 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2019

Which of the following processes can increase genetic diversity of bacteria in nature?
P. Conjugation
Q. Transformation
R. Transduction
S. Transfection

A

P only

B

P and Q only

C

P, Q and R only

D

P, Q, R and S

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19

2020 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2020

A list of pathogens (Group I) and a list of anti-microbial agents (Group II) used to treat their infections are given below. Match the pathogens with the corresponding anti-microbial agents.

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20

2021 · Biotechnology · General Biology · Microbiology

Biotechnology (BT) 2021

Coronavirus genome consists of

A

double-stranded DNA

B

double-stranded RNA

C

negative-sense single-stranded RNA

D

positive-sense single-stranded RNA

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