Upstream and Downstream Processing - Fundamentals of Biological Engineering - Biotechnology Previous Year Questions

1

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Protein A, which has strong affinity to Fc region of immunoglobulin, is extracted from

A

Saccharomyces cerevisiae

B

Staphylococcus aureus

C

Streptococcus pyogenes

D

Streptococcus sanguis

Open complete paper

2

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Which one of the following aminoacids in proteins does NOT undergo phosphorylation?

A

Ser

B

Thr

C

Pro

D

Tyr

Open complete paper

3

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

The role of an adjuvant isto

A

prolong the persistence of antigen

B

cross link the antigen

C

increase the size of antigen

D

avoid inflammation

Open complete paper

4

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Which one of the following is an ABC transporter?

A

multidrug resistance protein

B

acetylcholine receptor

C

bacteriorhodopsin

D

ATP synthase

Open complete paper

5

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

A callus of 5 g dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by 1.5 fold after 10 days of inoculation. The growth index of the culture is ________

Check answer

Open complete paper

6

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

A batch bioreactor is to be scaled up from 10 to 10,000 liters. The diameter of the large bioreactor is 10 times that of the small bioreactor. The agitator speed in the small bioreactor is 450 rpm. Determine the agitator speed (rpm) of the large bioreactor with same impeller tip speed as that of the small bioreactor. ________

Check answer

Open complete paper

7

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Match the commercial microbial sources in Group I with the products in Group II.

Group I	Group II
P. Corynebacteriumillium	1. 2,3-Butane di-ol
Q. Klebsiellaoxytoca	2. Poly-β-hydroxybutyric acid
R. Aspergillusniger	3. Glutamic acid
S. Alcaligeneseutrophus	4. Citric acid

A

P-3,Q-1,R-2,S-4

B

P-3,Q-1,R-4,S-2

C

P-1,Q-3,R-2,S-4

D

P-1,Q-3,R-4,S-2

Open complete paper

8

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Match the entries in the Group I with the elution conditions in Group II.

Group I	Group II
P. Ion-exchange chromatography	1. Isocratic solvent
Q. Hydrophobic column chromatography	2. Ampholytes
R. Gel filtration chromatography	3. Increasing gradient of salt
S. Chromatofocusing	4. Decreasing gradient of polarity

A

P-4,Q-1,R-2,S-3

B

P-4,Q-3,R-1,S-2

C

P-3,Q-4,R-1,S-2

D

P-3,Q-4,R-2,S-1

Open complete paper

9

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).

Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation.

Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

A

Both (a) and (r) are true and (r) is the correct reason for (a).

B

Both (a) and (r) are true but (r) is not the correct reason for (a).

C

is true but (r) is false.

D

is false but (r) is true.

Open complete paper

10

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by CNBr is ________
Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln

Check answer

Open complete paper

11

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Calculate the molarity (μM) of the resulting solution.

A

66.6

B

16.6

C

0.67

D

0.067

Open complete paper

12

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

If the dissociation constant for solute-adsorbent binding is \( K_D \), the retention time of the solute in a chromatography column

A

increases with increasing \(K_D \)

B

decreases with increasing \(K_D \)

C

passes through minimum with increasing \(K_D \)

D

is independent of \(K_D \)

Open complete paper

13

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

In a batch culture of Penicillium chrysogenum, the maximum penicillin synthesis occurs during the

A

lag phase

B

exponential phase

C

stationary phase

D

death phase

Open complete paper

14

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

Which of the following is employed for the repeated use of enzymes in bioprocesses?

A

polymerization

B

immobilization

C

ligation

D

isomerization

Open complete paper

15

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given \( N \) = rotations/time, \( D \)=diameter of impeller)

A

\(\pi ND \)

B

\(\pi N^2 D \)

C

\(\pi ND^2 \)

D

none of these

Open complete paper

16

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

The 5’ ends of the mature forms of the prokaryotic mRNAs and tRNAs are

A

a triphosphate group in mRNAs and a monophosphate group in tRNAs

B

triphosphate groups in both mRNAs and tRNAs

C

monophosphate groups in both mRNAs and tRNAs

D

a monophosphate group in mRNAs and a triphosphate group in tRNAs

Open complete paper

17

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to

A

remove large proteins

B

remove collagen only

C

remove mycoplasma and microorganisms

D

remove foaming agents

Open complete paper

18

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was gradually decreasing. Microbiological assays of reactor samples showed absence of contamination and recorded data showed no change in the operating conditions. The most probable reason for decrease in lysine concentration may be attributed to

A

accumulation of ethanol

B

growth of revertants

C

production of citric acid

D

unutilized phosphoenol pyruvate

Open complete paper

19

2014 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2014

If a plant is shifted to cold temperature, which of the following changes would take place in its membrane?

A

Ratio of unsaturated to saturated fatty acids would increase

B

Ratio of unsaturated to saturated fatty acids would decrease

C

Absolute amount of both fatty acids would increase keeping the ratio same

D

Absolute amount of both fatty acids would remain unchanged

Open complete paper

20

2016 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2016

ATP biosynthesis takes place utilizing the H⁺ gradient in mitochondria and chloroplasts. Identify the correct sites of H⁺ gradient formation.

A

Across the outer membrane of mitochondria and across the inner membrane of chloroplast

B

Across the inner membrane of mitochondria and across the thylakoid membrane of chloroplast

C

Within the matrix of mitochondria and across the inner membrane of chloroplast

D

Within the matrix of mitochondria and within the stroma of chloroplast

Open complete paper