Molecular Biology - Genetics, Cellular and Molecular Biology - Biotechnology Previous Year Questions

1

2013 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2013

Under alkaline conditions, DNA is more stable than RNA because

A

RNA forms secondary structures

B

RNA is a single stranded molecule

C

RNA has uracil in place of thymidine

D

RNA is susceptible to hydrolysis

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2

2013 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2013

Which one of the following modifications is common to both protein and DNA?

A

SUMOylation

B

Nitrosylation

C

Methylation

D

Ubiquitination

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3

2013 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2013

The RNA primer synthesized during the replication process in bacteria is removed by

A

DNA gyrase

B

primase

C

DNA polymerase I

D

DNA polymerase II

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4

2014 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2014

In a mouse genome, the numbers of functional Vα, Jα, Vβ, Dβ, Jβ gene segments are 79, 38, 21, 2 and 11, respectively. The total number of possible combinations for αβ T cell receptors are ____ ×10⁶.

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5

2016 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2016

A DNA sequence, 5'-ATGGACGTGCTTCCCAAAAGCATCGGGC-3', is mutated to obtain
P. 5' - ATGGACGTGCTTCaCAAAAGCATCGGGC- 3'
Q. 5' - ATGGACGTGCTTCCCgAAAGCATCGGGC- 3'
R. 5' - ATGGACGTGCTTCC -AAAGCATCGGGC- 3'
S. 5' - ATGGACGTGCTTCCCAAaGCATCGGGC- 3'
T. 5' - ATGGACgAGCTTCCCAAAGCATCGGGC- 3'
[Point mutations are shown in the lower case or ‘-’ within the sequences]
Which of the above mutant sequences DO NOT have frame-shift?

A

P, Q and S

B

P, S and T

C

Q, R and S

D

Q, S and T

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6

2012 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2012

In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by

A

acetylation

B

phosphorylation

C

methylation

D

glycosylation

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7

2012 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2012

Nude mice refers to

A

mice without skin

B

mice without thymus

C

knockout mice

D

transgenic mice

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8

2012 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2012

A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with

A

glycine

B

alanine

C

aspartate

D

threonine

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9

2012 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2012

A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?

A

Ribosomal protein gene

B

Transfer RNA gene

C

DNA repair gene

D

Ribosomal RNA gene

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10

2012 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2012

In zinc finger proteins, the amino acid residues that coordinate zinc are

A

Cys and His

B

Asp and Glu

C

Arg and Lys

D

Asp and Arg

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11

2012 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2012

The molar concentration of the repressor in the cell is

A

0.1 nM

B

1 nM

C

10 nM

D

100 nM

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12

2012 · Biotechnology · Genetics, Cellular and Molecular Biology · Molecular Biology

Biotechnology (BT) 2012

Therefore the lac-operon is

A

repressed and can only be induced with lactose.

B

repressed and cannot be induced with lactose.

C

not repressed.

D

expressed only when glucose and lactose are present.

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