Upstream and Downstream Processing - Fundamentals of Biological Engineering - Biotechnology Previous Year Questions

1

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

The role of an adjuvant isto

A

prolong the persistence of antigen

B

cross link the antigen

C

increase the size of antigen

D

avoid inflammation

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2

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Which one of the following is an ABC transporter?

A

multidrug resistance protein

B

acetylcholine receptor

C

bacteriorhodopsin

D

ATP synthase

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3

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

A callus of 5 g dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by 1.5 fold after 10 days of inoculation. The growth index of the culture is ________

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4

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

A batch bioreactor is to be scaled up from 10 to 10,000 liters. The diameter of the large bioreactor is 10 times that of the small bioreactor. The agitator speed in the small bioreactor is 450 rpm. Determine the agitator speed (rpm) of the large bioreactor with same impeller tip speed as that of the small bioreactor. ________

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5

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Match the commercial microbial sources in Group I with the products in Group II.

Group I	Group II
P. Corynebacteriumillium	1. 2,3-Butane di-ol
Q. Klebsiellaoxytoca	2. Poly-β-hydroxybutyric acid
R. Aspergillusniger	3. Glutamic acid
S. Alcaligeneseutrophus	4. Citric acid

A

P-3,Q-1,R-2,S-4

B

P-3,Q-1,R-4,S-2

C

P-1,Q-3,R-2,S-4

D

P-1,Q-3,R-4,S-2

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6

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Match the entries in the Group I with the elution conditions in Group II.

Group I	Group II
P. Ion-exchange chromatography	1. Isocratic solvent
Q. Hydrophobic column chromatography	2. Ampholytes
R. Gel filtration chromatography	3. Increasing gradient of salt
S. Chromatofocusing	4. Decreasing gradient of polarity

A

P-4,Q-1,R-2,S-3

B

P-4,Q-3,R-1,S-2

C

P-3,Q-4,R-1,S-2

D

P-3,Q-4,R-2,S-1

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7

2013 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2013

Calculate the molarity (μM) of the resulting solution.

A

66.6

B

16.6

C

0.67

D

0.067

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8

2019 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2019

The dimensions and operating condition of a lab-scale fermentor are as follows:
Volume = 1 L
Diameter = 20 cm
Agitator speed = 600 rpm
Ratio of impeller diameter to fermentor diameter = 0.3
This fermentor needs to be scaled up to 8,000 L for a large scale industrial application. If the scale-up is based on constant impeller tip speed, the speed of the agitator in the larger reactor is ______ rpm. Assume that the scale-up factor is the cube root of the ratio of fermentor volumes.

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9

2025 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2025

The contour length of a B-DNA molecule that encodes a bacterial protein of 33 kDa is ____ nm.
Consider the average molecular weight of an amino acid as 110 Da and helix rise per base pair for B-DNA as 0.34 nm.
(Round off to the nearest integer)

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10

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

A

it is an organic solvent

B

it easily penetrates cells

C

it protects cells by preventing crystallization of water

D

it is also utilized as a nutrient

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11

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

Heat inactivation of serum is done to inactivate

A

prions

B

mycoplasma

C

complement

D

pathogenic bacteria

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12

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

The molarity of water in a water : ethanol mixture (15 : 85, v/v) is approximately

A

0.85

B

5.55

C

8.5

D

55.5

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13

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

A single base pair of DNA weighs 1.1 × 10^-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 μg of purified DNA?

A

0.30

B

0.55

C

0.25

D

0.91

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14

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: Plants convert fatty acids into glucose.
Reason: Plants have peroxisomes.

A

Both (a) and (r) are true but (r) is not the correct reason for (a)

B

Both (a) and (r) are true and (r) is the correct reason for (a)

C

is true but (r) is false

D

is false but (r) is true

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15

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

Match the entries in Group I with the process parameters in Group II.

Group I	Group II
P. Clark electrode	1. Liquid level
Q. Redox probe	2. Dissolved oxygen concentration
R. Load cell	3. Vessel pressure
S. Diaphragm gauge	4. pH (anaerobic process)

A

P-2, Q-1, R-3, S-4

B

P-4, Q-2, R-3, S-1

C

P-2, Q-4, R-1, S-3

D

P-2, Q-1, R-4, S-3

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16

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

Match the downstream processes in Group I with the products in Group II.

Group I	Group II
P. Solvent extraction	1. Lactic acid
Q. Protein-A linked affinity chromatography	2. Penicillin
R. Extractive distillation	3. Monoclonal antibody
S. Salting out	4. Lipase

A

P-2, Q-3, R-1, S-4

B

P-4, Q-1, R-2, S-3

C

P-4, Q-1, R-3, S-2

D

P-2, Q-4, R-1, S-3

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17

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

Match the entries in Group I with the methods of sterilization in Group II.

Group I	Group II
P. Serum	1. Autoclave
Q. Luria broth	2. Membrane filtration
R. Polypropylene tubes	3. UV irradiation
S. Biological safety cabinets	4. Gamma irradiation
	5. Dry heat

A

P-5, Q-3, R-1, S-4

B

P-1, Q-4, R-5, S-3

C

P-2, Q-1, R-4, S-3

D

P-4, Q-1, R-3, S-5

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18

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

Match the high energy compounds in Group I with the biosynthetic pathways for the molecules in Group II.

Group I	Group II
P. GTP	1. Fatty acid
Q. UTP	2. Phospholipid
R. CTP	3. Protein
S. Acyl coenzyme A	4. Peptidoglycan

A

P-3, Q-2, R-4, S-1

B

P-2, Q-4, R-3, S-1

C

P-4, Q-3, R-1, S-2

D

P-3, Q-4, R-2, S-1

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19

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

A bacterial culture (200 μl containing 1.8 × 10⁶ cells) was treated with an antibiotic Z (50 μg per ml) for 4 h at 37 °C. After this treatment, the culture was divided into two equal aliquots.
Set A: 100 μl was plated on Luria agar.
Set B: 100 μl was centrifuged, the cell pellet washed and plated on Luria agar.
After incubating these two plates for 24 h at 37 °C, Set A plate showed no colonies, whereas the Set B plate showed 0.9 × 10⁵ cells. This experiment showed that the antibiotic Z is

A

bacteriostatic

B

bacteriocidal

C

bacteriolytic

D

apoptotic

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20

2012 · Biotechnology · Fundamentals of Biological Engineering · Upstream and Downstream Processing

Biotechnology (BT) 2012

The fold purification for each step is

A

P-0.1, Q- 0.66, R-0.84, S- 1.26

B

P-1.0, Q-0.52, R-0.67, S-0.8

C

P-1, Q-6.6, R-8.4, S-12.6

D

P-100, Q-66, R-84, S-12

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