Bioreaction Engineering - Fundamentals of Biological Engineering - Biotechnology Previous Year Questions

1

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

The catalytic efficiency for an enzyme is defined as

A

\(k_{cat}\)

B

\(\frac{V_{max}}{k_{cat}}\)

C

\(\frac{k_{cat}}{K_m}\)

D

\(\frac{k_{cat}}{V_{max}}\)

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2

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

A chemostat is operated at a dilution rate of \(0.6 h^{-1}\). At steady state, the biomass concentration in the exit stream was found to be \(30 g l^{-1}\). The biomass productivity (\(g l^{-1} h^{-1}\)) after 3h of steady state operation will be ________

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3

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

In a batch culture, the specific rate of substrate utilization is \(0.25 g (g cell mass)^{-1} h^{-1}\) and specific rate of product formation is \(0.215 g (g cell mass)^{-1} h^{-1}\). Calculate the yield of product from the substrate(\(Y_{p/s}\)). ________

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4

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).

Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation.

Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

A

Both (a) and (r) are true and (r) is the correct reason for (a).

B

Both (a) and (r) are true but (r) is not the correct reason for (a).

C

is true but (r) is false.

D

is false but (r) is true.

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5

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

The activity of an enzyme was measured by varying the concentration of the substrate (S) in the presence of three different concentrations of inhibitor (I) 0, 2 and 4 mM. The double reciprocal plot given below suggests that the inhibitor (I) exhibits

A

substrate inhibition

B

uncompetitive inhibition

C

mixed inhibition

D

competitive inhibition

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6

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

The maximum cell concentration (g l^-1) expected in a bioreactor with initial cell concentration of 1.75 g l^-1 and an initial glucose concentration of 125 g l^-1 is (Y_X/S = 0.6 g cell/g substrate) ________

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7

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

A fed batch culture was operated with intermittent addition of glucose solution at a flow rate of 200 ml h^-1. The values of K_s, μ_m and D are 0.3 g l^-1, 0.4 h^-1 and 0.1 h^-1, respectively. Determine the concentration of growth limiting substrate (gl^-1) in the reactor at quasi-steady state. ________

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8

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

The number of ligand binding sites present on receptors R1 and R3, respectively are

A

1 and 4

B

1 and 1

C

4 and 1

D

2 and 2

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9

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

Which one of the receptors has the highest affinity for the ligand?

A

R1

B

R2

C

R3

D

R4

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10

2013 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2013

What is the holding period (min) at a k value of 3.36min^-1?

A

10.6

B

9.5

C

8.4

D

7.2

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11

2019 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2019

Which one of the following can NOT be a limiting substrate if Monod's growth kinetics is applicable?

A

Extracellular carbon source

B

Extracellular nitrogen source

C

Dissolved oxygen

D

Intracellular carbon source

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12

2025 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2025

For the coupled reactions given below
Glucose 6-phosphate + H₂O → Glucose + Pi (Reaction 1)
ATP + Glucose → ADP + Glucose 6-phosphate (Reaction 2)
the standard free energy change of ATP hydrolysis at 25 °C is __________ kJ/mol.
The equilibrium constants for Reaction 1 and Reaction 2 are 360 and 800, respectively; Gas constant R = 8.314 J mol^-1 K^-1. (Round off to two decimal places)

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13

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

Choose the correct signal transduction pathway.

A

Hormone → 7 TM receptor → G protein → cAMP → PKA

B

Hormone → G protein → 7 TM receptor → cAMP → PKA

C

Hormone → 7 TM receptor → G protein → PKA → cAMP

D

Hormone → 7 TM receptor → cAMP → G protein → PKA

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14

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is

A

1.66 × 10⁴ IU

B

60 IU

C

6 × 10⁷ IU

D

10⁶ IU

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15

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

Identify the statement that is NOT applicable to an enzyme catalyzed reaction.

A

Enzyme catalysis involves propinquity effects

B

The binding of substrate to the active site causes a strain in the substrate

C

Enzymes do not accelerate the rate of reverse reaction

D

Enzyme catalysis involves acid-base chemistry

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16

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 g l^-1 (DCW), the specific growth rate (μ) is 0.4 h^-1 and substrate uptake rate (ν) is 16 g l^-1 h^-1. The cell yield coefficient Y_X/S will be

A

0.32

B

0.64

C

0.80

D

0.50

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17

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: Cell mass yield of a methylotrophic yeast is more on methanol compared to glucose.
Reason: Methanol has a greater degree of reductance compared to glucose.

A

Both (a) and (r) are correct and (r) is the correct reason for (a)

B

is correct, (r) is false

C

is false, (r) is correct

D

Both (a) and (r) are correct but (r) is not the correct reason for (a)

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18

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

An enzymatic reaction is described by the following rate expression.
\[ v = \frac{V_{max} s}{K_m + s + s^2/K_i} \]
Which one of the following curves represents this expression?

A

B

A bacterial culture (200 l containing 1.8 × \(10^{9}\) cells) was treated with an antibiotic Z (50 g

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19

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

The free energy change for the transport of three Na⁺ out of the cell is

A

+1.5 kJ/mol

B

+17.4 kJ/mol

C

+18.9 kJ/mol

D

+36.3 kJ/mol

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20

2012 · Biotechnology · Fundamentals of Biological Engineering · Bioreaction Engineering

Biotechnology (BT) 2012

The free energy change for the transport of two K⁺ into the cell is

A

+8.0 kJ/mol

B

+11.6 kJ/mol

C

+19.6 kJ/mol

D

+31.2 kJ/mol

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